Flowchart of representative AS variant (RASV) decision

We decided representative AS variants (RASVs) as following process (Figure 1): 1. Single-exon and 5'/3'-end truncated transcripts (see Scheme of truncated transcript decision) were removed from the loci of H-InvDB; 2. Genomic rearrangement gene families such as Immunoglobulin (Ig) and T-cell receptor (TCR) and an anomalistic high polymorphic gene family such as Major histocompatibility complex (MHC) were also removed; 3. AS were decided by whole pair-wise check of exon structure in the locus (see Scheme of RASV decision); 4. The AS variants were grouped if their genomic structures were same; 5. A representative was decided from each AS variant group by the priority of feasible ORF and the median of coverage on genome. It was defined as RASV (Figure 2).

Figure 1. Flowchart of RASV decision

Figure 2. Definition of RASV