H-InvDB x AHG DB
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H-Invitational ID: HIT000260083 Accession number: BC060781 Created date: 26-Mar-2013 Last modified: 27-May-2015
Definition: Mitochondrial Rho GTPase 1; MIRO-1; hMiro-1; EC=3.6.5.-; Rac-GTP-binding protein-like protein; Ras homolog gene family member T1;
 
 

Transcript original information
Accession number BC060781.1
CAGE tag ID NA
EST ID NA
Clone Number MGC:71555 IMAGE:4823115
Experimental resources NBRC: NITE Biological Resource Center  NBRC ; HGPD: Human Gene and Protein Database HGPD ; Antibody: searching human antibodies at "BIO-kaimono.com" Antibody (RHOT1) ; Catalog: searching experimental product catalogs at "BIO-kaimono.com" Catalog (RHOT1);
Sequence data provider NA
Annotation project NA
Length of cDNA 3148[bp] (No. of exon:19)[A:952 T:907 G:671 C:618]
Devision HUM
Molecular type mRNA
Library origin Cell type NA
Tissue type Testis
Develpmental stage NA
Mini-G
Sequence quality information
CDS feature Complete CDS
Kozak sequence NA
PolyA Site: 3133(+)
Vector/adapter sequence NA
Frame shift NA
Remaining intron NA
Splice site acceptor (NAGNAG) TAGAAG; 
Transcript quality feature NA
Notes NA

Gene structure information  G-integra H-DBAS cDNA-genome alignment
H-Inv cluster ID HIX0013701
Genomic location  G-integra Help Chromosome 17
Location 17q11.2
Position 30469473- 30552789
Strand +
Possible duplicated location(s) NA
Gene structure 19 exon(s)
Database links RefSeq NA
Ensembl NA
Entrez Gene Entrez Gene ID:55288
KEGG GENES KEGG GENES(55288)
GeneCard GeneCardRHOT1*GeneCards is provided free to academic non-profit institutions.
Related H-InvDB links H-DBASH-DBAS G-integraG-integra cDNA-genome alignmentcDNA-genome alignment

Predicted CDS information
HIP ID HIP000142218
Predicted CDS 240..2096;  618[aa];  Orientation:+3; 
Codon Adaptation Index (CAI). 0.703

Motif information
ORF

length(618),orf(240:2096)
MKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPER
VPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPL
INERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAK
NLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDN
DGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHISDGVADSGFTLKG
FLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTE
LNHHAYLFLQSTFDKHDLDRDCALSPDELKGLFKVFPYIPWGPDVNNTVC
TNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAVT
VTRDKKIDLQKKQTQRNVFRCNVIGVKNCGKSGVLQALLGRNLMRQKKIR
EDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAEIICDVVCLVYDVSN
PKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSISPTDFCRKHK
MPPPQAFTCNTADAPSKDIFVKLTTMAMYPHVTQADLKSSTFWLRASFGA
TVFAVLGFAMYKALLKQR*
a.a.
length
InterPro Name
length(177), motif(1:177) 177 IPR020860 MIRO [Domain]
length(169), motif(2:170) 169 IPR027417 P-loop containing nucleoside triphosphate hydrolase [Domain]
length(201), motif(2:202) 201 IPR027417 P-loop containing nucleoside triphosphate hydrolase [Domain]
length(160), motif(3:162) 160 IPR005225 Small GTP-binding protein domain [Domain]
length(22), motif(5:26) 22 IPR001806 Small GTPase superfamily [Family]
length(161), motif(6:166) 161 IPR001806 Small GTPase superfamily [Family]
length(14), motif(109:122) 14 IPR001806 Small GTPase superfamily [Family]
length(23), motif(144:166) 23 IPR001806 Small GTPase superfamily [Family]
length(90), motif(177:266) 90 IPR011992 EF-hand domain pair [Domain]
length(36), motif(184:219) 36 IPR002048 EF-hand domain [Domain]
length(29), motif(188:216) 29 IPR002048 EF-hand domain [Domain]
length(13), motif(197:209) 13 IPR018247 EF-Hand 1, calcium-binding site [Binding_site]
length(89), motif(218:306) 89 IPR013567 EF hand associated, type-2 [Domain]
length(36), motif(304:339) 36 IPR002048 EF-hand domain [Domain]
length(57), motif(308:364) 57 IPR011992 EF-hand domain pair [Domain]
length(29), motif(308:336) 29 IPR002048 EF-hand domain [Domain]
length(75), motif(340:414) 75 IPR013566 EF hand associated, type-1 [Domain]
length(150), motif(412:561) 150 IPR020860 MIRO [Domain]
length(164), motif(414:577) 164 IPR027417 P-loop containing nucleoside triphosphate hydrolase [Domain]
length(170), motif(415:584) 170 IPR027417 P-loop containing nucleoside triphosphate hydrolase [Domain]
length(111), motif(420:530) 111 IPR013684 Mitochondrial Rho-like [Domain]

Gene function information  Gene family PPI viewer Similarity Search Tool TACT
H-Inv ID HIT000260083
H-Inv cluster ID Locus viewHIX0013701
Accession number BC060781.1
CAGE tag ID NA
EST ID NA
Transcript feature  Help NO; 
Coding potential  Help Protein coding; 
Definition Mitochondrial Rho GTPase 1; MIRO-1; hMiro-1; EC=3.6.5.-; Rac-GTP-binding protein-like protein; Ras homolog gene family member T1;
Similarity category  Help Category: Identical to known human protein(Category I).
Identical to known human protein (Q8IXI2)  [Identity/coverage = 99.676%/100.0%] to Homo sapiens (Human). protein.
Experimental evidence Protein evidence
PubMed ID 11230166124828791269605914521508147020391548933416625196166305621952829821269460ALL
Gene family/group Gene family H-Inv gene family/group ID NA
Gene family/group name NA
Evidence motif (InterPro) ID NA
Gene symbol/name HGNC symbol RHOT1
HGNC aliases "ras homolog gene family, member T1"
HGNC name ras homolog family member T1
DDBJ RHOT1
UniProt RHOT1
EC number EC 3.6.5.-Acting on GTP; involved in cellular and subcellular movement; 
GGDB
(GlycoGene Database)
Gene symbol NA
Familly NA
Designation NA
Expression NA
KEGG metabolic pathway NA
Protein-protein interaction (PPI) PPI viewer H-Inv protein ID HIP000142218
No. of interaction 1
Interaction partner(s) HIP000084371
BIND NA
DIP NA
MINT NA
HPRD NA
IntAct NA
Database links RefSeq NA
Ensembl NA
Entrez Gene Entrez Gene ID:55288
KEGG GENES KEGG GENES(55288)
GeneCard GeneCardRHOT1*GeneCards is provided free to academic non-profit institutions.
etc H-GOLDHuman-Gene diversity Of Life-style related Diseases
Curation status Auto-annotated
Notes NA
Related H-InvDB links Gene familyGene family;  Similarity Search ToolSimilarity Search Tool;  TACTTACT
fRNAdb (The functional RNA database) : overlapping fRNAdb entries with H-InvDB transcripts based on the location of the genome. 
FR ID FR191362
Accession
Description
Location
PMID

Gene ontology information
Molecular function GTP binding (GO:0005525);  calcium ion binding (GO:0005509); 
Biological process small GTPase mediated signal transduction (GO:0007264); 
Cellular component intracellular (GO:0005622); 

Subcellular localization information  Last modified:27-May-2015
WoLF PSORT cytosol;  nuclear;  extracellular; 
Target P Other
SOSUI soluble protein
TMHMM membrane protein
PTS1 Not targeted
Related H-InvDB links LIFEdb LIFEdb; 
JRE-1.4.0 or later is required. Download JRE at Sun's web site.

Protein structure information (GTOP) GTOP Last modified:27-May-2015
Start End PDB_ID E-value Identity Coverage SCOP_ID
16 171 1e6vB1 2e-18 16.0 144/253 a.89.1.1
177 334 1xo5A 6e-13 16.9 154/174 a.39.1.5
309 367 1wlzA1 3e-05 20.3 59/83 a.39.1.7
419 552 1a2kC 1e-06 11.9 126/196 c.37.1.8
544 580 1ndbA2 4e-04 30.4 23/220 c.43.1.3
Related H-InvDB links GTOP GTOP

Gene expression information  H-ANGEL DNAProbeLocator Last modified:27-May-2015
Tissue-specific expression  H-ANGEL NA
Probe
information DNAProbeLocator
AceGene AGhsA150404;  AGhsB240808; 
Affymetrix
GeneChip
HG-Focus NA
HG-U133 218323_at;  222148_s_at; 
HG-U133A 218323_at;  222148_s_at; 
HG-U133A_2 218323_at;  222148_s_at; 
HG-U133B NA
HG-U133_Plus_2 218323_at;  222148_s_at; 
HG-U95 91428_at; 
HG-U95A NA
HG-U95B NA
HG-U95C NA
HG-U95D NA
HG-U95E 91428_at; 
HG-U95Av2 NA
HuEx-1_0 3503427;  3503428;  3503429;  3503430;  3503431;  3503433;  3717540;  3717541;  3717542;  3717545;  3717548;  3717549;  3717550;  3717551;  3717555;  3717556;  3717558;  3717559;  3717560;  3717561;  3717562;  3717563;  3717564;  3717566;  3717567;  3717568;  3717570;  3717572;  3717576;  3717588;  3717589;  3717590;  3717591;  3717592;  3717593;  3752582;  3780495;  3780496;  3925286;  3925287;  3925288;  3925290; 
HuGeneFL NA
Agilent Human 1A Oligo Microarray:PGID215 A_23_P89492; 
Whole Human Genome Oligo Microarray:PGID247 A_24_P59765; 
Related H-InvDB links H-ANGELH-ANGEL DNAProbeLocatorDNAProbeLocator

Polymorphism (SNP, indel), microsatellite (Short Tandem Repeat, STR) and repeat information  VaryGene Repeat mask viewer
 Single Nucleotide Polymorphism (SNP) and indel  VaryGene
Location Variation dbSNP ID Strand CDS/UTR Translation
390 .. 390 G/A rs145143892 + CDS Nonsynonymous[Val51Ile]
431 .. 431 T/A rs199735465 + CDS Nonsynonymous[Ser64Arg]
605 .. 605 G/T rs201893717 + CDS Synonymous[Leu122Leu]
652 .. 652 A/G rs116363165 + CDS Nonsynonymous[Tyr138Cys]
713 .. 713 C/T rs147576979 + CDS Synonymous[Leu158Leu]
722 .. 722 C/T rs201021051 + CDS Synonymous[Tyr161Tyr]
869 .. 869 C/T rs141985401 + CDS Synonymous[Asn210Asn]
929 .. 929 G/A rs145734789 + CDS Synonymous[Lys230Lys]
1005 .. 1005 A/T rs28630420 + CDS Nonsynonymous[Thr256Ser]
1057 .. 1057 G/A rs116192944 + CDS Nonsynonymous[Arg273Gln]
1076 .. 1076 C/T rs149000823 + CDS Synonymous[Asp279Asp]
1103 .. 1103 C/- rs111248500 + CDS
1190 .. 1190 T/C rs115949021 + CDS Synonymous[Asp317Asp]
1285 ^ 1286 -/T rs34538349 + CDS
1364 .. 1364 G/A rs114899192 + CDS Synonymous[Gln375Gln]
1421 .. 1421 T/G rs57831260 + CDS Synonymous[Ser394Ser]
1423 .. 1423 A/G rs141923086 + CDS Nonsynonymous[Gln395Arg]
1442 .. 1442 G/T rs201011889 + CDS Synonymous[Val401Val]
1474 .. 1474 A/C rs199972893 + CDS Nonsynonymous[Lys412Thr]
1697 .. 1697 A/G rs16967164 + CDS Synonymous[Glu486Glu]
1711 .. 1711 T/C rs138851743 + CDS Nonsynonymous[Val491Ala]
1730 .. 1730 T/C rs191457576 + CDS Synonymous[Asp497Asp]
1750 .. 1750 T/C rs80181477 + CDS Nonsynonymous[Phe504Ser]
1784 .. 1784 T/A rs141986224 + CDS Nonsynonymous[Phe515Leu]
1831 .. 1831 T/G rs146295337 + CDS Nonsynonymous[Leu531Arg]
1854 .. 1854 A/G rs139664057 + CDS Nonsynonymous[Ser539Gly]
1918 .. 1918 A/G rs149321179 + CDS Nonsynonymous[Asn560Ser]
1930 .. 1930 C/G rs199545262 + CDS Nonsynonymous[Ala564Gly]
1982 .. 1982 C/T rs114672765 + CDS Synonymous[His581His]
2011 .. 2011 C/T rs147592052 + CDS Nonsynonymous[Thr591Met]
2023 .. 2023 G/A rs140387858 + CDS Nonsynonymous[Arg595Gln]
2051 .. 2051 A/G rs58010811 + CDS Synonymous[Ala604Ala]
2330 .. 2330 G/T rs114795322 + 3'UTR
2361 .. 2361 G/A rs7207471 + 3'UTR
2440 .. 2440 G/C rs149609981 + 3'UTR
2590 .. 2590 G/A rs190500838 + 3'UTR
2655 .. 2655 C/T rs180926692 + 3'UTR
2656 .. 2656 G/A rs147312326 + 3'UTR
2796 ^ 2797 -/A rs143886333 + 3'UTR
2873 .. 2873 C/T rs185000418 + 3'UTR
2876 .. 2876 A/T rs139249661 + 3'UTR
2954 .. 2954 C/T rs16967203 + 3'UTR
3090 .. 3090 A/T rs80273262 + 3'UTR
 Microsatellite (Short Tandem Repeat, STR)
No data available
 Microsatellite: Human-Gene diversity Of Life-style related Diseases (H-GOLD)
No data available
 Repeat  Repeat mask viewer
No data available
Database links H-GOLDHuman-Gene diversity Of Life-style related Diseases(H-GOLD)
Related H-InvDB links VaryGeneVaryGene;  Repeat mask viewerRepeat Mask Viewer